Common Protocols

Explore the Common Protocols for your Species

BEEF CATTLE

Protocol 1 (4 handlings)

The most common protocol used for beef cattle in Australia. This protocol is based on research and yields the most reliable results.

Features:

  • Requires cattle to be handled 4 times.

  • Reliable protocol – generally yields best results.

Purpose:

  • Synchronising cows/heifers (Bos taurus) for FTAI. Heat detection can also be incorporated into the protocol if desired.

  • Synchronising recipient cows/heifers (Bos taurus) for transfer of either ET or IVF embryos.

 

 

Download PDF:

 

Protocol 2 (3 handlings)

Typically used in commercial FTAI protocols where breeders prefer to limit the number of handlings.

Features:

  • Requires cattle to be handled 3 times.

  • Research shows that this protocol could yield a result up to 5% less than protocol 1. When this protocol is used, it is accepted due to the benefits in reduced handling.

  • Although this protocol works okay in heifers, where possible prefer to use protocol 1.

  • A good choice when:

    • Handling cows and calves to reduce issues with mustering.

    • Holding paddocks are not close to yards or easily mustered.

Purpose:

  • Synchronising cows (Bos taurus) for FTAI. Heat detection can also be incorporated into the protocol is desired.

  • When heat detection is used in conjunction with FTAI:

    • Heat detect and AI from evening Day 8 to morning Day 10 using AM/PM rule (no need for GnRH injection in these animals).

    • Day 10, any females that have not been submitted to AI, FTAI as per schedule and administer GnRH.

 

 

Download PDF:

 

Protocol 2 (Heifers – Afternoon) 

Commonly used protocol for HEIFERS when it is appropriate to AI in the AFTERNOON.

The most common protocol used for Bos indicus heifers. Backed by research and considered to be the ‘best practice’ protocol.

Features:

Purpose:

  • Synchronising heifers for FTAI. Heat detection can also be incorporated into the protocol if desired.

  • Synchronising recipient heifers for transfer of either ET or IVF embryos.

 

 

Download PDF:

 

Protocol 3 (Heifers – Morning)

A new protocol for HEIFERS when it is preferred to AI in the MORNING to avoid the heat or to better organise your day.

Specifically designed for use in Bos indicus heifers. Research has demonstrated that an increase in pregnancy rates may result when using this protocol [7,8].

Features:

Purpose:

  • Synchronising heifers for FTAI. Heat detection can also be incorporated into the protocol if desired.

  • Synchronising recipient heifers for transfer of either ET or IVF embryos.

 

 

Download PDF:

 

Where possible, we avoid recommending protocols that utilise GnRH in cattle that have Bos indicus content.

Protocol 1 (Cows)

The typical protocol used for COWS with any Bos indicus content.

The most common protocol used for beef cattle in Australia. This protocol is based on research and yields the most reliable results.

Features:

  • Requires cattle to be handled 4 times.

  • Reliable protocol – generally yields best results.

Purpose:

  • Synchronising cows for FTAI. Heat detection can also be incorporated into the protocol if desired.

  • Synchronising recipient cows for transfer of either ET or IVF embryos.

 

 

Download PDF:

 

J-Synch

Features:

  • Ideal for beef herds that are not EU accredited.
  • This protocol features an extended length between removal of Cue-Mate® device and the time of ovulation, which has been associated with optimal fertility.
  • eCG may be administered at Day 6 if desired.
  • No requirement for heat detection.

Purpose:

  • Synchronisation of heifers for FTAI

Synchronisation of recipient heifers for transfer of either ET or IVF embryos

 

 

Download PDF:

 

BEEF CATTLE - EU COMPLIANT

Simple FTAI

Protocols for those that wish to take the simple approach to enable FTAI.

Features:

  • Morning AI activities for all types of cattle.
  • Cows require two injections of PG on Day 5 (at least 8 hrs apart) to ensure full regression of the CL, which is necessary for ovulation to occur.
  • Protocols are appropriate for both Bos taurus and Bos indicus cattle.
  • eCG may be administered at Day 5 if desired.
  • No requirement for heat detection.

Purpose:

  • Synchronisation of cows/heifers for FTAI
  • Synchronisation of recipient cows/heifers for transfer of either ET or IVF embryos

 

 

Download PDF:

Read the brochure here.

 

Simple FTAI

Protocols for those that wish to take the simple approach to enable FTAI.

Features:

  • Morning AI activities for all types of cattle.
  • Cows require two injections of PG on Day 5 (at least 8 hrs apart) to ensure full regression of the CL, which is necessary for ovulation to occur.
  • Protocols are appropriate for both Bos taurus and Bos indicus cattle.
  • eCG may be administered at Day 5 if desired.
  • No requirement for heat detection.

Purpose:

  • Synchronisation of cows/heifers for FTAI
  • Synchronisation of recipient cows/heifers for transfer of either ET or IVF embryos

 

 

Download PDF:

Read the brochure here.

 

FTAI with heat detection

Protocols for those that want to enjoy the benefits of FTAI, but are happy to spend a little more time to get the best result per straw of semen used.

Features:

  • Cows require two injections of PG on Day 5 (at least 8 hrs apart) to ensure full regression of the CL, which is necessary for ovulation to occur.
  • Protocols are appropriate for both Bos taurus and Bos indicus cattle.
  • eCG may be administered at Day 5 if desired.
  • Heat detection aids (Tail Paint, Estrotect or Kamar) are used to select heifers/cows for first round of FTAI. No need to visually observe heifers/cows in standing heat.
  • Option to use sexed, rare, or more expensive semen in heifers/cows that have been identified on heat, by heat detection aids for first round of FTAI. These animals are likely to have a higher pregnancy rate to AI.

Purpose:

  • Synchronisation of cows/heifers for FTAI where resources are sufficient to handle the cattle more frequently.
  • When breeders are seeking a higher pregnancy rate per straw of semen.

 

 

Download PDF:

Read the brochure here.

 

FTAI with heat detection

Protocols for those that want to enjoy the benefits of FTAI, but are happy to spend a little more time to get the best result per straw of semen used.

Features:

  • Cows require two injections of PG on Day 5 (at least 8 hrs apart) to ensure full regression of the CL, which is necessary for ovulation to occur.
  • Protocols are appropriate for both Bos taurus and Bos indicus cattle.
  • eCG may be administered at Day 5 if desired.
  • Heat detection aids (Tail Paint, Estrotect or Kamar) are used to select heifers/cows for first round of FTAI. No need to visually observe heifers/cows in standing heat.
  • Option to use sexed, rare, or more expensive semen in heifers/cows that have been identified on heat, by heat detection aids for first round of FTAI. These animals are likely to have a higher pregnancy rate to AI.

Purpose:

  • Synchronisation of cows/heifers for FTAI where resources are sufficient to handle the cattle more frequently.
  • When breeders are seeking a higher pregnancy rate per straw of semen.

 

 

Download PDF:

Read the brochure here.

 

You can find the PDF brochure version of the EU protocols here.

DAIRY CATTLE

Protocol 2 – Cue-Mate + Oestradiol (4 handlings)

A commonly used protocol. The use of oestradiol benzoate is permitted in heifers.

Features:

  • Requires cattle to be handled 4 times.

  • Reliable protocol – generally yields best results.

Purpose:

  • Synchronising heifers for FTAI. Heat detection can also be incorporated into the protocol if desired.

  • Synchronising heifers for transfer of either ET or IVF embryos.

 

 

Download PDF:

 

Protocol 3 – Cue-Mate + Oestradiol (3 handlings)

Typically used where producers prefer to limit the number of handlings.

Features:

  • Requires cattle to be handled 3 times.

  • This protocol could yield a result up to 5% less than protocol 1. When this protocol is used, it is accepted due to the benefits in reduced handling.

Purpose:

  • Synchronising heifers for FTAI. Heat detection can also be incorporated into the protocol is desired.

  • When heat detection is used in conjunction with FTAI:

    • Heat detect and AI from evening Day 8 to morning Day 10 using AM/PM rule (no need for GnRH injection in these animals).

    • Day 10, any females that have not been submitted to AI, FTAI as per schedule and administer GnRH.

 

 

Download PDF:

 

Protocol 1 – Cue-Mate + OvSynch

The OvSync protocol is a well-known protocol for synchronising cows in dairy herds. However, it can be unreliable producing variable results. When including a Cue-Mate® into an OvSynch protocol there are numerous benefits.

Features:

  • Improves the synchrony of ovulation compared to a basic OvSynch program – more reliable results.

  • Treats NVO cows in advance, getting these cows in calf earlier.

  • Can treat some reproductive disorders.

  • Can be used in heifers.

Purpose:

  • Synchronising cows/heifers for FTAI. Heat detection can also be incorporated into the protocol if desired.

  • Synchronising recipient cows/heifers for transfer of either ET or IVF embryos.

  • If using in heifers, dose of eCG is 300 IU.

 

 

Download PDF:

 

DONOR COWS

Protocol 4 – Donor Cows

Donor cow protocols will vary considerably. The dose of FSH is tailored according to each female. We strongly recommend that you utilise a protocol that is prescribed by your ET technician.

An example of a typical protocol to superovulate and synchronise a donor cow is listed below. The protocol features the use of a Cue-Mate®, when used in conjunction with oestradiol benzoate has tight control over the follicular wave. This enables a targeted use of the FSH to ensure that an optimal superovulatory response.

 

 

Download PDF:

 

SHEEP

AI

Protocol 1 – Ewes

Protocols to synchronise ovulation in ewes are relatively simple. It is highly recommended that you follow the prescribed protocol from your veterinarian. Your veterinarian may choose to alter the duration of progesterone insertion time, or the dose of eCG. This could depend on progesterone source used, the season, the breed, weight or body condition of the ewes. A typical protocol is listed below:

 

 

Download PDF:

 

Protocol 2 – Donor Ewes

Donor ewe protocols may vary considerably. We strongly recommend that you utilise a protocol that is prescribed by your ET technician.

An example of a typical protocol to superovulate and synchronise a donor ewe is listed below.

 

 

Download PDF:

 

KEY: ODB: oestradiol benzoate (1 mg/ml) PG: Prostaglandin (cloprostenol; 250 μg/ml) GnRH: Gonadotrophin releasing hormone (Gonadorellin; 100 μg/ml) Pregnecol: Equine chorionic gonadotrophin (200 IU/ml) Cue-Mate®: Intravaginal progesterone device (1.56 g progesterone) 1-pod Cue-Mate®: (0.78 g progesterone).